Skip to content

2011-03-05 new articles we read this week

Microbiology

  1. Dubey GP, Ben-Yehuda S. Intercellular nanotubes mediate bacterial communication. Cell. 2011 Feb 18;144(4):590-600. PubMed PMID: 21335240.
    • A new way of bacterial communication is discovered.

Genetics/ Genomics

  1. Takashima Y, Ohtsuka T, González A, Miyachi H, Kageyama R. Intronic delay is essential for oscillatory expression in the segmentation clock. Proc Natl Acad Sci U S A. 2011 Feb 7. [Epub ahead of print] PubMed PMID: 21300886.
  2. Nikolova EN, Kim E, Wise AA, O’Brien PJ, Andricioaei I, Al-Hashimi HM. Transient Hoogsteen base pairs in canonical duplex DNA. Nature. 2011 Feb 24;470(7335):498-502. Epub 2011 Jan 26. PubMed PMID: 21270796.
    • Commentary: Honig B, Rohs R. Biophysics: Flipping Watson and Crick. Nature. 2011 Feb 24;470(7335):472-3. PubMed PMID: 21350476.
    • A possible 3D configuration of DNA is in equilibrium with the regular DNA, thus expanding the variety of the possible DNA recognition.
  3. Kedes L, Liu E, Jongeneel CV, Sutton G. Judging the Archon Genomics X PRIZE for whole human genome sequencing. Nat Genet. 2011 Mar;43(3):175. PubMed PMID: 21350493.
  4. Towards a medical grade human genome sequence. Nat Genet. 2011 Mar;43(3):173.  PubMed PMID: 21350492.

Development

  1. Manceau M, Domingues VS, Mallarino R, Hoekstra HE. The developmental role of Agouti in color pattern evolution. Science. 2011 Feb 25;331(6020):1062-5. PubMedPMID: 21350176.

Retinal development

  1. Kerschensteiner D. Circuit assembly: the repulsive side of lamination. Curr Biol. 2011 Feb 22;21(4):R163-6. PubMed PMID: 21334299.
  2. Whitney IE, Raven MA, Lu L, Williams RW, Reese BE. A QTL on Chromosome 10 Modulates Cone Photoreceptor Number in the Mouse Retina. Invest Ophthalmol Vis Sci. 2011 Feb 17. [Epub ahead of print] PubMed PMID: 21330668.

Neurodevelopment

  • A series of brainbow applications and modifications!
  1. Card JP, Kobiler O, McCambridge J, Ebdlahad S, Shan Z, Raizada MK, Sved AF, Enquist LW. Microdissection of neural networks by conditional reporter expression from a Brainbow herpesvirus. Proc Natl Acad Sci U S A. 2011 Feb 3. [Epub ahead of print] PubMed PMID: 21292985.
  2. Cachero S, Jefferis GS. Double brainbow. Nat Methods. 2011 Mar;8(3):217-8. PubMed PMID: 21358624.
  3. Hampel S, Chung P, McKellar CE, Hall D, Looger LL, Simpson JH. Drosophila Brainbow: a recombinase-based fluorescence labeling technique to subdivide neural expression patterns. Nat Methods. 2011 Mar;8(3):253-9. Epub 2011 Feb 6. PubMed PMID: 21297621.
  4. Hadjieconomou D, Rotkopf S, Alexandre C, Bell DM, Dickson BJ, Salecker I. Flybow: genetic multicolor cell labeling for neural circuit analysis in Drosophila melanogaster. Nat Methods. 2011 Mar;8(3):260-6. Epub 2011 Feb 6. PubMed PMID: 21297619.

Regeneration

  1. Porrello ER, Mahmoud AI, Simpson E, Hill JA, Richardson JA, Olson EN, Sadek HA. Transient regenerative potential of the neonatal mouse heart. Science. 2011 Feb 25;331(6020):1078-80. PubMed PMID: 21350179.

Cdk5

  1. Sato K, Minegishi S, Takano J, Plattner F, Saito T, Asada A, Kawahara H, Iwata N, Saido TC, Hisanaga SI. Calpastatin, an endogenous calpain-inhibitor protein, regulates the cleavage of the Cdk5 activator p35 to p25. J Neurochem. 2011 Feb 21. doi: 10.1111/j.1471-4159.2011.07222.x. [Epub ahead of print] PubMed PMID: 21338355.

Ethical, Social and Legal Issues

  1. Ceci SJ, Williams WM. Understanding current causes of women’s underrepresentation in science. Proc Natl Acad Sci U S A. 2011 Feb 7. [Epub ahead of print] PubMed PMID: 21300892.
  2. Muggah R. Measuring the true costs of war: consensus and controversy. PLoS Med. 2011 Feb 15;8(2):e1000417. PubMed PMID: 21358809; PubMed Central PMCID: PMC3039686.
  3. Hicks MH, Dardagan H, Guerrero Serdán G, Bagnall PM, Sloboda JA, Spagat M. Violent deaths of iraqi civilians, 2003-2008: analysis by perpetrator, weapon, time, and location. PLoS Med. 2011 Feb 15;8(2):e1000415. PubMed PMID: 21358813; PubMed Central PMCID: PMC3039690.

Research Funding

  1. Cho A. U.S. science budget. House cuts to DOE national labs would also hamstring industry. Science. 2011 Feb 25;331(6020):997-8. PubMed PMID: 21350135.

2011-02-27 New articles we read this week

From the news

Genomics

  • Xu Z, Wei W, Gagneur J, Clauder-Münster S, Smolik M, Huber W, Steinmetz LM. Antisense expression increases gene expression variability and locus interdependency. Mol Syst Biol. 2011 Feb 15;7:468. PubMed PMID: 21326235.
  • Tewhey R, Bansal V, Torkamani A, Topol EJ, Schork NJ. The importance of phase information for human genomics. Nat Rev Genet. 2011 Mar;12(3):215-223. Epub 2011 Feb 8. PubMed PMID: 21301473.

Development

  • Wu D, Freund JB, Fraser SE, Vermot J. Mechanistic Basis of Otolith Formation during Teleost Inner Ear Development. Dev Cell. 2011 Feb 15;20(2):271-8. PubMed PMID: 21316594.
  • Davidson EH. Evolutionary bioscience as regulatory systems biology. Dev Biol. 2011 Feb 11. [Epub ahead of print] PubMed PMID: 21320483.

Retina

  • Jusuf PR, Almeida AD, Randlett O, Joubin K, Poggi L, Harris WA. Origin and determination of inhibitory cell lineages in the vertebrate retina. J Neurosci. 2011 Feb 16;31(7):2549-62. PubMed PMID: 21325522.

Gene regulatory network

  • Macneil L, Walhout AJ. Gene regulatory networks and the role of robustness and stochasticity in the control of gene expression. Genome Res. 2011 Feb 4. [Epub ahead of print] PubMed PMID: 21324878.
  • Taati S, Formenti E, Comet JP, Bernot G. On the impact of the distance between two genes on their interaction curve. J Math Biol. 2011 Feb 15. [Epub ahead of print] PubMed PMID: 21327639.

Systems biology

  • Plotkin JB. Cell biology. The lives of proteins. Science. 2011 Feb 11;331(6018):683-4. PubMed PMID: 21310990.
  • Eden E, Geva-Zatorsky N, Issaeva I, Cohen A, Dekel E, Danon T, Cohen L, Mayo A, Alon U. Proteome half-life dynamics in living human cells. Science. 2011 Feb 11;331(6018):764-8. Epub 2011 Jan 13. PubMed PMID: 21233346.
  • Minc N, Burgess D, Chang F. Influence of cell geometry on division-plane positioning. Cell. 2011 Feb 4;144(3):414-26. PubMed PMID: 21295701.
  • Gibson WT, Veldhuis JH, Rubinstein B, Cartwright HN, Perrimon N, Brodland GW, Nagpal R, Gibson MC. Control of the mitotic cleavage plane by local epithelial topology. Cell. 2011 Feb 4;144(3):427-38. PubMed PMID: 21295702.

Stem cells

  • Bock C, Kiskinis E, Verstappen G, Gu H, Boulting G, Smith ZD, Ziller M, Croft GF, Amoroso MW, Oakley DH, Gnirke A, Eggan K, Meissner A. Reference Maps of Human ES and iPS Cell Variation Enable High-Throughput Characterization of Pluripotent Cell Lines. Cell. 2011 Feb 4;144(3):439-52. PubMed PMID: 21295703.

Ken Robinson says schools kill creativity

Just watched this over lunch. A very good speech.

U.S. Biggest Budget Cuts in History

It has always been my role to tell you the reality and teach you how to fish, and not giving you a fish, nor the bogus “American Dream” or things-will-somehow-get-better hope. I have been saying for a while that the economy is going to be much worse and now the really hard time is going to come.

The House approved one of the biggest cuts in US history. When they were discussing this, there were many scientists trying to oppose to the plan, including FASEB. Why? Look at the numbers, among the comprehensive cuts in all governmental agencies, here are a few that we are very familiar with:

NSF   -$139M

CDC   -$755M

NIH   -$1B

One billion cut at NIH means that the funding rate will become even lower and many programs will not be sustained. As they also put it

Make no mistake, these cuts are not low-hanging fruit.  These cuts are real and will impact every District across the country”.

Put it simply, it is a survival game. There is nowhere to hide in society now given the budget cut, poor fundamentals of the US economy and societal structure. Those who want to get a chance (note: it is “getting a chance”) to survive need to show their very best in this modernized jungle. You need to fight very hard for your chance and survival. If you possess the “American-Dream” mentality that “I have done everything and I have tried, hence I deserve an “A” or “X”, despite things didn’t work out”, you will have trouble in this game.

 

2011-02-19 articles we read this week

Human Genome

  1. Lander ES. Initial impact of the sequencing of the human genome. Nature. 2011 Feb 10;470(7333):187-97. PubMed PMID: 21307931.
      Eric is one of the leaders in the human genome sequencing project. In this review that celebrates the publication of human genome sequence 10 years ago, he highlights major discoveries at all levels, from how we understand gene, genome structure and regulation, to how we understand
      disease, map disease genes, find better treatments and to the evolution of human kind. This is an article that you can’t miss if you are serious about studying biology.
  2. Mardis ER. A decade’s perspective on DNA sequencing technology. Nature. 2011 Feb 10;470(7333):198-203. PubMed PMID: 21307932.
  3. Green ED, Guyer MS; National Human Genome Research Institute. Charting a course for genomic medicine from base pairs to bedside. Nature. 2011 Feb 10;470(7333):204-13. PubMed PMID: 21307933.

Evolution and Human Behavior

  1. Kermyt G. Anderson. Does paying child support reduce men’s subsequent marriage and fertility? Evol Hum Behav. 2011; 32: 90-96
  2. Drew H. Bailey, Kristina M. Durante, David C. Geary. Men’s perception of women’s attractiveness is calibrated to relative mate value and dominance of the women’s partner. Evol Hum Behav. 2011; 32: 138-146
  3. Jeffrey K. Snyder, Daniel M.T. Fessler, Leonid Tiokhin, David A. Frederick, Sok Woo Lee, Carlos David Navarrete. Trade-offs in a dangerous world: women’s fear of crime predicts preferences for aggressive and formidable mates. Evol Hum Behav. 2011; 32: 127-137
  4. Dina Dosmukhambetova, Antony Manstead. Strategic reactions to unfaithfulness: female self-presentation in the context of mate attraction is linked to uncertainty of paternity. Evol Hum Behav. 2011; 106-117.

Stem cells

  1. Wadman M. Stem cells: The crusader. Nature. 2011 Feb 10;470(7333):156-9. PubMed PMID: 21307911.

Retina

  1. Matsuoka RL, Nguyen-Ba-Charvet KT, Parray A, Badea TC, Chédotal A, Kolodkin AL. Transmembrane semaphorin signalling controls laminar stratification in the mammalian retina. Nature. 2011 Jan 26;470(7333):259-263. [Epub ahead of print] PubMed PMID: 21270798.

2011-02-12 new articles we read this week

Systems biology

  1. Kim J, Winfree E. Synthetic in vitro transcriptional oscillators. Mol Syst Biol. 2011 Feb 1;7:465. PubMed PMID: 21283141.
  2. Montagne K, Plasson R, Sakai Y, Fujii T, Rondelez Y. Programming an in vitro DNA oscillator using a molecular networking strategy. Mol Syst Biol. 2011 Feb 1;7:466. PubMed PMID: 21283142.
  3. Bachman JA, Sorger P. New approaches to modeling complex biochemistry. Nat Methods. 2011 Feb;8(2):130-1. PubMed PMID: 21278724.
  4. Sneddon MW, Faeder JR, Emonet T. Efficient modeling, simulation and coarse-graining of biological complexity with NFsim. Nat Methods. 2011 Feb;8(2):177-83. Epub 2010 Dec 26. PubMed PMID: 21186362.

Genetics

  1. Clarke GM, Anderson CA, Pettersson FH, Cardon LR, Morris AP, Zondervan KT. Basic statistical analysis in genetic case-control studies. Nat Protoc. 2011 Feb;6(2):121-33. Epub 2011 Feb 3. PubMed PMID: 21293453.

Zebrafish

  1. Blackburn JS, Liu S, Raimondi AR, Ignatius MS, Salthouse CD, Langenau DM. High-throughput imaging of adult fluorescent zebrafish with an LED fluorescence macroscope. Nat Protoc. 2011 Feb;6(2):229-41. Epub 2011 Feb 3. PubMed PMID: 21293462.

Development & Regeneration

  1. Diep CQ, Ma D, Deo RC, Holm TM, Naylor RW, Arora N, Wingert RA, Bollig F, Djordjevic G, Lichman B, Zhu H, Ikenaga T, Ono F, Englert C, Cowan CA, Hukriede NA, Handin RI, Davidson AJ. Identification of adult nephron progenitors capable of kidney regeneration in zebrafish. Nature. 2011 Feb 3;470(7332):95-100. Epub 2011 Jan 26. PubMed PMID: 21270795.
  2. Spence JR, Mayhew CN, Rankin SA, Kuhar MF, Vallance JE, Tolle K, Hoskins EE, Kalinichenko VV, Wells SI, Zorn AM, Shroyer NF, Wells JM. Directed differentiation of human pluripotent stem cells into intestinal tissue in vitro. Nature. 2011 Feb 3;470(7332):105-9. Epub 2010 Dec 12. PubMed PMID: 21151107; PubMed Central PMCID: PMC3033971.

Imaging

  1. Bewersdorf J, Farese RV Jr, Walther TC. A new way to look at fat. Nat Methods. 2011 Feb;8(2):132-3. PubMed PMID: 21278725.
  2. Lionnet T, Czaplinski K, Darzacq X, Shav-Tal Y, Wells AL, Chao JA, Park HY, de Turris V, Lopez-Jones M, Singer RH. A transgenic mouse for in vivo detection of endogenous labeled mRNA. Nat Methods. 2011 Feb;8(2):165-70. Epub 2011 Jan 16. PubMed PMID: 21240280.

2011-02-05 New articles we read this week

Lab life

  1. The care and maintenance of your adviser

Neuroscience

  1. Lau BY, Mathur P, Gould GG, Guo S. Identification of a brain center whose activity discriminates a choice behavior in zebrafish. Proc Natl Acad Sci U S A. 2011 Jan 24;. [Epub ahead of print] PMID: 21262817 [PubMed – as supplied by publisher]
  2. Tay TL, Ronneberger O, Ryu S, Nitschke R, Driever W. Comprehensive catecholaminergic projectome analysis reveals single-neuron integration of zebrafish ascending and descending dopaminergic systems. Nat Commun. 2011Jan;2:171. PubMed PMID: 21266970.
  3. Gräff J, Tsai LH. Cognitive enhancement: A molecular memory booster. Nature. 2011 Jan 27;469(7331):474-5. PubMed PMID: 21270879.
  4. Chen DY, Stern SA, Garcia-Osta A, Saunier-Rebori B, Pollonini G, Bambah-Mukku D, Blitzer RD, Alberini CM. A critical role for IGF-II in memory consolidation and enhancement. Nature. 2011 Jan 27;469(7331):491-7. PubMed PMID: 21270887.

Zebrafish

  1. Gerety SS, Wilkinson DG. Morpholino artifacts provide pitfalls and reveal a novel role for pro-apoptotic genes in hindbrain boundary development. Dev Biol. 2010 Dec 8. [Epub ahead of print] PubMed PMID: 21145318.

Genomics

  1. Majewski J, Pastinen T. The study of eQTL variations by RNA-seq: from SNPs to phenotypes. Trends Genet. 2010 Nov 29. [Epub ahead of print] PubMed PMID: 21122937.
  2. Zhang Z, Pugh BF. High-Resolution Genome-wide Mapping of the Primary Structure of Chromatin. Cell. 2011 Jan 21;144(2):175-86. PubMed PMID: 21241889.

Gene regulatory network

  1. Chu DF, Zabet NR, Hone AN. Optimal Parameter Settings for Information Processing in Gene Regulatory Networks. Biosystems. 2011 Jan 20. [Epub ahead of print] PubMed PMID: 21256918.
  2. Novershtern N, Subramanian A, Lawton LN, Mak RH, Haining WN, McConkey ME, Habib N, Yosef N, Chang CY, Shay T, Frampton GM, Drake AC, Leskov I, Nilsson B, Preffer F, Dombkowski D, Evans JW, Liefeld T, Smutko JS, Chen J, Friedman N, Young RA, Golub TR, Regev A, Ebert BL. Densely interconnected transcriptional circuits control cell States in human hematopoiesis. Cell. 2011 Jan 21;144(2):296-309. PubMed PMID: 21241896.
  3. Grundberg E, Adoue V, Kwan T, Ge B, Duan QL, Lam KC, Koka V, Kindmark A, Weiss ST, Tantisira K, Mallmin H, Raby BA, Nilsson O, Pastinen T. Global Analysis of the Impact of Environmental Perturbation on cis-Regulation of Gene Expression. PLoS Genet. 2011 Jan 20;7(1):e1001279. PubMed PMID: 21283786; PubMed Central PMCID: PMC3024267

Ethical, Social and Legal Issues

  1. Bredenoord AL, Kroes HY, Cuppen E, Parker M, van Delden JJ. Disclosure of individual genetic data to research participants: the debate reconsidered. Trends Genet. 2010 Dec 27. [Epub ahead of print] PubMed PMID: 21190750.

Eye disease

  1. Baye LM, Patrinostro X, Swaminathan S, Beck JS, Zhang Y, Stone EM, Sheffield VC, Slusarski DC.  The N-terminal region of CEP290 restores vision in a zebrafish model of human blindness. Hum Mol Genet. 2011 Jan 21;. [Epub ahead of print]

Disease

  1. Liu S, Leach SD. Zebrafish models for cancer. Annu Rev Pathol. 2011 Feb 28;6:71-93. PubMed PMID: 21261518.

Brg1 & Wnt

  1. Griffin CT, Curtis CD, Davis RB, Muthukumar V, Magnuson T. The chromatin-remodeling enzyme BRG1 modulates vascular Wnt signaling at two levels.  Proc Natl Acad Sci U S A. 2011 Jan 24. [Epub ahead of print] PubMed PMID: 21262838.

Cell biology

  1. Ikeda K, Semenova I, Zhapparova O, Rodionov V. Melanophores for microtubule dynamics and motility assays. Methods Cell Biol. 2010;97:401-14. Review. PubMed PMID: 20719282.
  2. Pagh-Roehl K, Wang E, Burnside B. Posttranslational modifications of tubulin in teleost photoreceptor cytoskeletons. Cell Mol Neurobiol. 1991 Dec;11(6):593-610. PubMed PMID: 1782652.

Funding

  1. Macilwain C. Funding in 2011: East heats up as west cools down. Cell. 2011 Jan 21;144(2):167-9. PubMed PMID: 21241886.

Horizon: Science Under Attack

A nice documentary by Paul Nurse, a Nobel laureates in 2001 for his work on cell cycle, on public skepticism on scientific findings.

There are a number of interesting articles with the opposite view, some even from the people who were interviewed in the documentary.

In paricluar, James Delingpole

of course there are also people who liked the documentary

2011-01-29 New articles we read this week

Neuroscience

  1. Miller G. Social neuroscience. Why loneliness is hazardous to your health. Science. 2011 Jan 14;331(6014):138-40. PubMed PMID: 21233358.

Genomics

  1. Ozsolak F, Milos PM. RNA sequencing: advances, challenges and opportunities. Nat Rev Genet. 2011 Feb;12(2):87-98. Epub 2010 Dec 30. PubMed PMID: 21191423.
  2. Bravo HC, Irizarry RA. Model-based quality assessment and base-calling for second-generation sequencing data. Biometrics. 2010 Sep;66(3):665-74. doi: 10.1111/j.1541-0420.2009.01353.x. Review. PubMed PMID: 19912177; PubMed Central PMCID: PMC2888717.
  3. Taub MA, Corrada Bravo H, Irizarry RA. Overcoming bias and systematic errors in next generation sequencing data. Genome Med. 2010 Dec 10;2(12):87. [Epub ahead of print] PubMed PMID: 21144010.
  4. Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, Magen A, Canidio E, Pagani M, Peluso I, Lin-Marq N, Koch M, Bilio M, Cantiello I, Verde R, De Masi C, Bianchi SA, Cicchini J, Perroud E, Mehmeti S, Dagand E, Schrinner S, Nürnberger A, Schmidt K, Metz K, Zwingmann C, Brieske N, Springer C, Hernandez AM, Herzog S, Grabbe F, Sieverding C, Fischer B, Schrader K, Brockmeyer M,
    Dettmer S, Helbig C, Alunni V, Battaini MA, Mura C, Henrichsen CN, Garcia-Lopez R, Echevarria D, Puelles E, Garcia-Calero E, Kruse S, Uhr M, Kauck C, Feng G, Milyaev N, Ong CK, Kumar L, Lam M, Semple CA, Gyenesei A, Mundlos S, Radelof U, Lehrach H, Sarmientos P, Reymond A, Davidson DR, Dollé P, Antonarakis SE, Yaspo ML, Martinez S, Baldock RA, Eichele G, Ballabio A. A high-resolution anatomical atlas of the transcriptome in the mouse embryo. PLoS Biol. 2011 Jan 18;9(1):e1000582. PubMed PMID: 21267068.

Genome-Wide Association Study

  1. Shields R. Common disease: are causative alleles common or rare? PLoS Biol. 2011 Jan 18;9(1):e1001009. PubMed PMID: 21267067.
  2. Goldstein DB. The importance of synthetic associations will only be resolved empirically. PLoS Biol. 2011 Jan 18;9(1):e1001008. PubMed PMID: 21267066.
  3. Anderson CA, Soranzo N, Zeggini E, Barrett JC. Synthetic associations are unlikely to account for many common disease genome-wide association signals. PLoS Biol. 2011 Jan 18;9(1):e1000580. PubMed PMID: 21267062.
  4. Wray NR, Purcell SM, Visscher PM. Synthetic Associations Created by Rare Variants Do Not Explain Most GWAS Results. PLoS Biol. 2011 Jan 18;9(1):e1000579. PubMed PMID: 21267061.

Systems Biology

  1. Snijder B, Pelkmans L. Origins of regulated cell-to-cell variability. Nat Rev Mol Cell Biol. 2011 Feb;12(2):119-25. Epub 2011 Jan 12. PubMed PMID: 21224886.

Retina & Retinal Pigment Epithelium

  1. Choi RY, Engbretson GA, Solessio E, Jones GA, Coughlin A, Aleksic I, Zuber ME. Cone Degeneration Following Rod Ablation in a Reversible Model of Retinal Degeneration. Invest Ophthalmol Vis Sci. 2010 Aug 18. [Epub ahead of print] PubMed PMID: 20720220.
  2. Dwyer MA, Kazmin D, Hu P, McDonnell DP, Malek G. Research Resource: Nuclear Receptor Atlas of Human Retinal Pigment Epithelial Cells: Potential Relevance to Age-Related Macular Degeneration. Mol Endocrinol. 2011 Jan 14. [Epub ahead of print] PubMed PMID: 21239617.

Zebrafish

  1. Sánchez-Vázquez FJ, Terry MI, Felizardo VO, Vera LM. Daily Rhythms of Toxicity and Effectiveness of Anesthetics (MS222 and Eugenol) in Zebrafish (Danio Rerio). Chronobiol Int. 2011 Mar.;28(2):109-117. PubMed PMID: 21231872.

Medicine

  1. The Lancet. Religion, organ transplantation, and the definition of death. Lancet. 2011 Jan 22;377(9762):271. PubMed PMID: 21256368.

Dedicated Purdue students who worked in darkness to bring lights to the world

Getting ready!

Caleb: I saw it I saw it I saw it! Jin: No, you don't.

Zeran: I can work hard even in the dark! I can work hard even without eating! I can work hard even without drinking! I can work hard even without sleeping! I can... I can ... I can... who am I?