Leung Lab Blog

Retinal development

1. Masuda T, Zhang X, Berlinicke C, Wan J, Yerrabelli A, Conner EA, Kjellstrom S, Bush R, Thorgeirsson SS, Swaroop A, Chen S, Zack DJ. The Transcription Factor GTF2IRD1 Regulates the Topology and Function of Photoreceptors by Modulating Photoreceptor Gene Expression across the Retina. J Neurosci. 2014 Nov 12;34(46):15356-68.
2. Okabe K, Kobayashi S, Yamada T, Kurihara T, Tai-Nagara I, Miyamoto T,
   Mukouyama YS, Sato TN, Suda T, Ema M, Kubota Y. Neurons limit angiogenesis by titrating VEGF in retina. Cell. 2014 Oct 23;159(3):584-96. doi: 10.1016/j.cell.2014.09.025. PubMed PMID: 25417109.

Retina

1. Baehr W. Membrane Protein Transport in Photoreceptors: The Function of PDEd.
   Invest Ophthalmol Vis Sci. 2014 Dec 30;55(12):8653-66. doi: 10.1167/iovs.14-16066. PubMed PMID: 25550383.

Genome editing

1. Auer TO, Duroure K, Concordet JP, Del Bene F. CRISPR/Cas9-mediated conversion of eGFP- into Gal4-transgenic lines in zebrafish. Nat Protoc. 2014 Dec;9(12):2823-40. doi: 10.1038/nprot.2014.187. Epub 2014 Nov 13. PubMed PMID: 25393779.
2. Yang L, Grishin D, Wang G, Aach J, Zhang CZ, Chari R, Homsy J, Cai X, Zhao Y, Fan JB, Seidman C, Seidman J, Pu W, Church G. Targeted and genome-wide sequencing reveal single nucleotide variations impacting specificity of Cas9 in human stem cells. Nat Commun. 2014 Nov 26;5:5507. doi: 10.1038/ncomms6507. PubMed PMID: 25425480.
3. Nielsen AA, Voigt CA. Multi-input CRISPR/Cas genetic circuits that interface host regulatory networks. Mol Syst Biol. 2014 Nov 24;10:763. doi:
   10.15252/msb.20145735. PubMed PMID: 25422271.
4. Byrne SM, Ortiz L, Mali P, Aach J, Church GM. Multi-kilobase homozygous
   targeted gene replacement in human induced pluripotent stem cells. Nucleic Acids Res. 2014 Nov 20. pii: gku1246. [Epub ahead of print] PubMed PMID: 25414332.
5. Naito Y, Hino K, Bono H, Ui-Tei K. CRISPRdirect: software for designing
   CRISPR/Cas guide RNA with reduced off-target sites. Bioinformatics. 2014 Nov 20. pii: btu743. [Epub ahead of print] PubMed PMID: 25414360.
6. Doudna JA, Charpentier E. Genome editing. The new frontier of genome
   engineering with CRISPR-Cas9. Science. 2014 Nov 28;346(6213):1258096. doi: 10.1126/science.1258096. PubMed PMID: 25430774.

Imaging

1. Paquet D, Plucinska G, Misgeld T. In vivo imaging of mitochondria in intact
   zebrafish larvae. Methods Enzymol. 2014;547:151-64. PubMed PMID: 25416357.

Zebrafish

1. McCluskey BM, Postlethwait JH. Phylogeny of zebrafish, a ‘model species’, within Danio, a ‘model genus’. Mol Biol Evol. 2014 Nov 20. pii: msu325. [Epub ahead of print] PubMed PMID: 25415969.
2. Magdeldin S, Blaser RE, Yamamoto T, Yates JR 3rd. Behavioral and Proteomic Analysis of Stress Response in Zebrafish (Danio rerio). J Proteome Res. 2014 Nov 26. [Epub ahead of print] PubMed PMID: 25398274.

Stem cells

1. Byrne SM, Ortiz L, Mali P, Aach J, Church GM. Multi-kilobase homozygous
   targeted gene replacement in human induced pluripotent stem cells. Nucleic Acids Res. 2014 Nov 20. pii: gku1246. [Epub ahead of print] PubMed PMID: 25414332.

Development & Disease

1. The Deciphering Developmental Disorders Study; The Deciphering Developmental Disorders Study. Large-scale discovery of novel genetic causes of developmental disorders. Nature. 2014 Dec 24. doi: 10.1038/nature14135. [Epub ahead of print] PubMed PMID: 25533962.

Genetics

1. Tan AS, Baty JW, Dong LF, Bezawork-Geleta A, Endaya B, Goodwin J, Bajzikova M, Kovarova J, Peterka M, Yan B, Pesdar EA, Sobol M, Filimonenko A, Stuart S, Vondrusova M, Kluckova K, Sachaphibulkij K, Rohlena J, Hozak P, Truksa J, Eccles D, Haupt LM, Griffiths LR, Neuzil J, Berridge MV. Mitochondrial Genome Acquisition Restores Respiratory Function and Tumorigenic Potential of Cancer Cells without Mitochondrial DNA. Cell Metab. 2015 Jan 6;21(1):81-94. doi: 10.1016/j.cmet.2014.12.003. PubMed PMID: 25565207.
   • Press release: Extraordinary gene transfer between cells observed

Medicine

1. Ling LL, Schneider T, Peoples AJ, Spoering AL, Engels I, Conlon BP, Mueller A, Schäberle TF, Hughes DE, Epstein S, Jones M, Lazarides L, Steadman VA, Cohen DR, Felix CR, Fetterman KA, Millett WP, Nitti AG, Zullo AM, Chen C, Lewis K. A new antibiotic kills pathogens without detectable resistance. Nature. 2015 Jan 7. doi: 10.1038/nature14098. [Epub ahead of print] PubMed PMID: 25561178.
   • Commentary: Wright G. Antibiotics: An irresistible newcomer. Nature. 2015 Jan 7. doi: 10.1038/nature14193. [Epub ahead of print] PubMed PMID: 25561172.
   • Nature news: Promising antibiotic discovered in microbial ‘dark matter’

Evolution

1. Yoshida MA, Yura K, Ogura A. Cephalopod eye evolution was modulated by the acquisition of Pax-6 splicing variants. Sci Rep. 2014 Mar 5;4:4256. doi: 10.1038/srep04256. PubMed PMID: 24594543; PubMed Central PMCID: PMC3942700.